Navigation und Service

ESBL and (fluoro)quinolone - Resistance in Enterobacteriaceae (RESET 2)

01/2014-12/2016

This third-party funded project is conducted in the framework of the BfR research programme on exposure estimation and assessment of biological risks.

BMBF grant number: 01KI1313B

Project homepage: www.reset-verbund.de

Project description:

The basis for the overall assessment of the risk of developing and transmitting antimicrobial resistances can be built only by linking detailed information on isolate properties with data of epidemiological and experimental studies. Here, (routine) standard phenotyping, (rapid) extended molecular methods as well as (deep) sequencing will be implemented as technologies to pinpoint transmission mechanisms and to provide diagnostic techniques for a routine follow-up for risk assessment. Following the overall hypotheses, the general concept of the second funding period of the RESET consortium can be sub-divided into three work packages (WP), namely "Selection" (WP1), "Transmission" (WP2) and "Colonisation and Disease" (WP3), respectively. These general work packages will be sub-divided into two parts, namely "Microbiology and Typing" (subsection a) and "Epidemiology and identification of intervention measures" (subsections b/c). In subsection a) bacterial resistance mechanisms and their impact on colonisation and infection will be characterised. In subsections b) and c) the relationship between exposure and selection in experimental settings as well as the major factors that have impact on spread of the pathogens will be highlighted. Moreover, these latter two subsections aim at finding starting points for intervention measures in the several steps of the food production chain to reduce the likelihood of infections for humans. As in the first funding period, these work packages have to be supplemented by work packages on "Data analysis and risk assessment" (WP 4) and "Consortium management" (WP 5).

BfR parts of the project:

Individual project (IP) 1 Molecular epidemiology of extended-spectrum β-lactamases (ESBL), AmpC β-lactamases and carbapenemases in Enterobacteriaceae from healthy food-producing animals, their environment and their resulting food-products with emphasis on emerging carbapenem resistance: The main objective of the IP1 study was and is to identify and characterise ESBL-producing S. enterica and E. coli isolates from all over Germany originating from healthy animals (mainly poultry and swine) and their food products, as well as the environment. The sources for the isolates were S. enterica and E. coli strains collected by passive screening, national and European monitoring programs, by the NRL of the BfR (S. enterica, E. coli and Antimicrobial Resistance).

IP2 Quantification of spread and transmission of extended-spectrum β-lactamase (ESBL)-, AmpC β-lactamase-, carbapenemase- or PMQR-producing Enterobacteriaceae and assessment of the related risk for public health: different sources and pathways may contribute to the exposure of humans with antimicrobially resistant microorganisms and to the public health impact related to this exposure. A risk assessment concept applicable to the German situation is being developed  in order to evaluate the human health impact of antimicrobial use of 3rd and 4th generation cephalosporins in animal production.

Project partners:

• Charité - Universitätsmedizin Berlin, Germany
• Friedrich-Loeffler-Institut, Germany
• Freie Universität Berlin, Germany
• Robert Koch-Institut, Germany
• University of Veterinary Medicine Hannover, Foundation, Germany
• University Hospital of Giessen and Marburg, Germany

Up